Figure 12

A representative dual, inducible piggyBac vector is functional as an insect transformation vector. D. melanogaster was transformed with pXLBacII-TetO1.GalT/GnTII-DsRed.A cl 3, which encodes GalT and GnTII under the control of the P _CMVmin -TetO7-P _CMVmin dual tetracycline-inducible transcriptional element, and several transgenic lines were isolated, as described in Methods. After being cultured in the presence or absence of doxycycline, larvae from five transgenic lines or wild type were extracted and the extracts were used to measure GnTII (A) and GalT (B) activities, as described in Methods. Each bar represents the average results obtained using duplicate samples of extracts from groups of larvae obtained from each fly line. The error bars show the standard deviations and the asterisks mark the lines that had statistically significant (p = 0.05) differences in enzyme activity levels measured in the absence and presence of doxycycline. The statistical analysis represents the results of one-tailed student's t-tests with the assumption of equal variances, which was checked by comparing the ratio of the variances with the appropriate F value on a table of F values for p = 0.05. The equal variance assumption failed for the GnTII (panel A) assays on lines #13 and #14 and for the GalT assay on line #13. Thus, those t-tests were repeated assuming unequal variance and these results are reported. The p values obtained for the GnTII assays (A) were #1 (0.26), #7 (0.01), #8 (0.01), #13 (0.05), and #14 (0.10). The p values obtained for the GalT assays (B) were #1 (0.00), #7 (0.01), #8 (0.19), #13 (0.03), and #14 (0.27).