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Figure 11 | BMC Biotechnology

Figure 11

From: Construction and characterization of new piggyBac vectors for constitutive or inducible expression of heterologous gene pairs and the identification of a previously unrecognized activator sequence in piggyBac

Figure 11

A representative dual, constitutive piggyBac vector is functional as an insect transformation vector. D. melanogaster was transformed with pXLBacII-CMP-SAS/SAS-EYFP.LTR-F cl 25, which encodes CMP-SAS and SAS under the control of the ie1-hr5-ie1 dual constitutivetranscriptional control element, and several transgenic lines were isolated, as described in Methods. After being cultured in the presence of N-acetylmannosamine, larvae from five transgenic lines or wild type were extracted and the extracts were used to measure total CMP-sialic acid (A) and sialic acid (B) contents, as described in Methods. Each bar represents the average results obtained using duplicate samples of extracts from groups of larvae obtained from each fly line.

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BMC Biotechnology

ISSN: 1472-6750

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