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Figure 3 | BMC Biotechnology

Figure 3

From: A new enzymatic route for production of long 5'-phosphorylated oligonucleotides using suicide cassettes and rolling circle DNA synthesis

Figure 3

Gel-based visualization of the oligonucleotide SF-WT90 amplified using the suicide cassette principle. Lane 1: Linear probe (SF-WT90).Lane 2: Circularized probe ((+)-strand). Note the incomplete ligation and the background smear. Lane 3: Circularized probe from lane 2 amplified by rolling circle DNA synthesis. Lane 4: Nicked RC1-product (Nt. Alw I). Lane 5: Cleaved RC1-product (Mly I). Lane 7: Same as lane 4. Lane 8: Circularized probe ((-)-strand). Note the apparently complete ligation and the absence of a background smear. Lane 9: Circularized probe from lane 8 amplified by rolling circle DNA synthesis. Lane 10: Nicked RC2-product (Nt. Alw I). Lane 11: Cleaved RC2-product (Mly I).Lane 6, 12 and 13: Size markers. Primers used: Pr SF-WT90 (+) and Pr SF-WT90 (-). The gel was stained with SYBR Gold.

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