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Figure 2 | BMC Biotechnology

Figure 2

From: A new enzymatic route for production of long 5'-phosphorylated oligonucleotides using suicide cassettes and rolling circle DNA synthesis

Figure 2

Example of the sequence composition of a suicide cassette. The suicide cassette contains a binding site (green) and a cleavage site (indicated by a vertical arrow) for the nicking enzyme Nt. Alw I and a binding site (blue) and a cleavage site (indicated by two vertical arrows) for the restriction endonuclease Mly I. Furthermore, the cassette comprises a loop structure (yellow) and extra bases for improving the cleavage and ligation steps (red). Top: Linear sequence. Bottom: Base pairing in a hairpin structure.

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BMC Biotechnology

ISSN: 1472-6750

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