Figure 1
From: One-step selection of Vaccinia virus-binding DNA aptamers by MonoLEX
Schematic presentation of MonoLEX in comparison to SELEX. Based on a combinatory oligonucleotide library, SELEX comprises several cycles of target binding, elution and amplification of putative aptamers. In contrast, MonoLEX starts with one affinity chromatography to sort non-binding oligonucleotides, low-affinity aptamers and high-affinity aptamers. Highly affine aptamers are amplified once and characterized further by an aptamer blot assay.