Figure 9

Real time Reverse Transcription-Polymerase Chain Reaction determination of light responsiveness of Cab19. Selfed seed from transgenic lines A85 (pBINCab19::GUSA) and C103 (pBINmas35S::GUS) and the untransformed control line, AC1, were germinated in the dark. Half the germinated seedlings from each line were transferred into the light and half were kept in the dark for 48 h. RNA extracted from the epicotyls of individual seedlings that tested positive for GUS served as templates for real time Reverse Transcription-Polymerase Chain Reactions. Results are reported on a relative scale as a percentage of C103 (light exposed seedling 1:L1) for each primer pair [native tomato Cab primers and GUS primers]. C-L: AC1 control seedling exposed to 48 h light; L1-L4: four different seedlings from the C103 transformed line on plates exposed to 48 h light; L9-12: four different seedlings from the A85 transformed line on plates exposed to 48 h light; C-D: AC1 control seedling kept in the dark for 48 h; D5-D8: four different seedlings from the C103 transformed line on plates that remained in the dark for 48 h; D13-D16: four different seedlings from the A85 line on plates that remained in the dark for 48 h. Note difference in scale for GUS RNA levels in A85 seedlings.