Table 1 Primers. List of oligonucleotides used for vector construction and Fc region cloning.
From: Construction of miniantibodies for the in vivo study of human autoimmune diseases in animal models
Number | Name | Orientation | Sequence |
|---|---|---|---|
A | Hygro-XbaI-inv | Antisense | AGCTTCTAGACAGCTTGGGTCTCCCTATAG |
B | Hygro-HindIII-inv | Sense | AGCTAAGCTTAAACCCGCTGATCAGC |
1 | 1-XbaI-leader | Sense | CAGGCGTCTAGATGCCACCATGGGCTGGAGCCTGATCCTCCTGTTCCTCGTCGCTGTGGCTACAGGTAAGGG |
2 | 2-leader-intron | Sense | TGTGGCTACAGGTAAGGGGCTCACAGTAGCAGGCTTGAGGTCTGGACATATATATGGGTGACAATGACATCCAC |
3 | 3-BssHII-scFv | Sense | GGTGACAATGACATCCACTTTGCCTTTCTCTCCACAGGTGGCGCGCATGCCGACATCCGGTTGACCCAG |
4 | scFv-NheI-HindIII | Antisense | CCGCTAAGCTTCGCCTGGCTAGCAAAAGCGTCCGTCGTATC |
5 | HuGCH2-s | Sense | AGGCGGCTAGCGACAAAACTCACACATGCCCACCGTGCCCA |
6 | HuGCH3-SV5-a | Antisense | CTGCTAAGCTTTTAAGTACTATCCAGGCCCAGCAGTGGGTTTGGGATTGGTTTGCCACTAGTTTTACCCGGGGACAGGGAGAG |
7 | HuACH2-s | Sense | CAGGCGGCT AGCGTTCCCTCAACTCCACCTACC |
8 | HuACH3-a | Antisense | CCGCTACTAGTTTTACCCGCCAAGCGGTCGAT |
9 | MoGCH3-a | Antisense | CCGCTACTAGTTTTACCAGGAGAGTGGGAGAG |
10 | MoGCH2-s | Sense | CAGGCG GCT AGC GGTTGTAAGCCTTGCATATGTACA |
11 | RaGCH3-a | Antisense | CCGCTACTAGTTTTACCCGGAGGCCGGGAGATG |
12 | RaGCH2-s | Sense | CAGGCG GCT AGC CACAAATGCCCTACATGCCCT |