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Figure 4 | BMC Biotechnology

Figure 4

From: Construction of miniantibodies for the in vivo study of human autoimmune diseases in animal models

Figure 4

Complement fixation assay with the miniantibodies constructs MB-HuG-2.8 and 3.7, MB-MoG-2.8 and 3.7, MB-RaG-2.8 and 3.7. The binding of C1q to the miniantibodies is revealed with biotinylated anti-C1q and streptavidin conjugated with alkaline phosphatase. Positive and negative control are represented by the murine anti-His D8 (IgG2a) and CUB7402 (IgG1) mAb, both recognizing the coated tTG.

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