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Table 3 Constructs, soluble expression and purification results of 57 proteins used for testing DDBP method

From: Domain selection combined with improved cloning strategy for high throughput expression of higher eukaryotic proteins

Well         
Row Column Accession Number Start position End position Length (aa) Protein definition Soluble expression level (18°C) Soluble expression level (37°C) Purified
A 2 NP_493355 1 300 300 C01A2.5 medium high yes
A 3 NP_001022737 1 264 264 X-box Binding Protein homolog family member (xbp-1) medium high not
A 7 NP_498947 1 282 282 PeRoXisome assembly factor family member (prx-19) high high Yes
A 9 NP_497226 1 253 253 W06E11.4 medium medium not
A 10 T26925 1 195 195 hypothetical protein Y45F10C.5 medium not soluble not
A 11 NP_495146 1 218 218 K05F1.9 medium high not
B 1 NP_495062 1 210 210 Helix Loop Helix family member (hlh-26) high high not
B 2 NP_496422 1 225 225 B0491.3 high low Yes
B 3 NP_495475 1 197 240 F10E7.2 not soluble low not
B 4 NP_496547 21 284 284 W03C9.1 medium high not
B 5 NP_496156 29 184 184 R53.8 low high not
B 8 NP_501161 1 250 250 F42C5.3 medium high Yes
B 9 NP_502163 1 319 319 C10C6.3 high high Yes
B 10 NP_500772 55 348 368 ZK354.6 not soluble not soluble not
B 11 NP_501789 1 297 297 F25H8.1 high high Yes
C 1 NP_501895 1 294 294 R09E10.1 medium high Yes
C 2 NP_500890 1 243 243 H32C10.2 high high not
C 3 NP_501981 1 388 388 R102.5a low low not
C 4 NP_507039 1 196 196 F14H3.5 high low not
C 5 NP_506094 1 183 183 F23H12.3 medium low not
C 6 NP_506094 1 90 183 F23H12.3 not soluble medium Yes
C 8 NP_505964 20 260 260 T04F3.2 medium medium not
C 9 NP_506495 1 252 252 D1086.4 high high not
C 10 NP_741113 1 394 419 C32A3.3a not soluble not soluble not
C 11 NP_501199 1 299 299 F55G1.9 not soluble low not
D 1 NP_495021 1 197 197 EEED8.12 high high not
D 6 NP_502315 1 199 199 F35G2.2 high high Yes
D 10 NP_491869 1 232 232 MeDiaTor family member (mdt-18) medium low not
E 1 NP_501936 1 190 190 F01D4.5b medium low not
E 2 NP_506929 26 144 206 F57A10.4 not soluble low not
E 3 NP_506929 26 206 206 F57A10.4 not soluble not soluble not
E 5 NP_491210 1 249 249 T12F5.1 high high not
E 7 NP_506245 35 240 240 R186.3 low not soluble not
E 8 NP_495941 1 269 308 T24H10.1 high medium Yes
E 10 NP_510298 1 269 269 AMP-Activated Kinase Beta subunit family member (aakb-1) medium low not
F 1 NP_492285 1 239 239 F02E9.5 medium low not
F 2 NP_509787 1 195 195 F13E6.1 medium high not
F 3 AAZ82857 1 230 230 Hypothetical protein C17H12.13 not soluble not soluble not
F 4 NP_493382 1 210 210 Y87G2A.10 high medium Yes
F 5 NP_497990 1 214 214 C38D4.9 high high Yes
F 10 NP_498391 1 217 217 C56G2.15 high high Yes
G 2 NP_493230 1 183 183 W02A11.2 high not soluble Yes
G 3 NP_492005 1 189 189 F22D6.2 high high Yes
G 4 NP_492692 1 206 206 Y106G6E.4 high medium Yes
G 5 NP_492795 1 207 207 C34B2.5 high medium Yes
G 6 NP_491736 1 214 214 C06A5.2 medium high not
G 7 NP_491358 1 233 233 ZK973.9 low medium not
G 8 NP_492301 1 240 240 D1081.9 not soluble not soluble not
G 9 NP_492301 1 65 240 D1081.9 high high Yes
G 11 NP_491721 1 273 273 B0207.11 high high not
H 2 NP_491965 1 274 274 T21G5.4 high low Yes
H 3 NP_491348 1 287 287 Y47D9A.2a not soluble not soluble not
H 4 NP_491903 27 330 363 D2092.4 high medium not
H 5 NP_496803 1 183 183 F15D4.2 medium low not
H 6 NP_491434 1 177 177 C10H11.7 low low not
H 8 NP_495527 30 179 179 F45E12.5b high high Yes
H 9 NP_494315 1 276 276 F22E5.8 not soluble not soluble not