Table 3 Constructs, soluble expression and purification results of 57 proteins used for testing DDBP method
Well | Â | Â | Â | Â | Â | Â | Â | Â | |
|---|---|---|---|---|---|---|---|---|---|
Row | Column | Accession Number | Start position | End position | Length (aa) | Protein definition | Soluble expression level (18°C) | Soluble expression level (37°C) | Purified |
A | 2 | NP_493355 | 1 | 300 | 300 | C01A2.5 | medium | high | yes |
A | 3 | NP_001022737 | 1 | 264 | 264 | X-box Binding Protein homolog family member (xbp-1) | medium | high | not |
A | 7 | NP_498947 | 1 | 282 | 282 | PeRoXisome assembly factor family member (prx-19) | high | high | Yes |
A | 9 | NP_497226 | 1 | 253 | 253 | W06E11.4 | medium | medium | not |
A | 10 | T26925 | 1 | 195 | 195 | hypothetical protein Y45F10C.5 | medium | not soluble | not |
A | 11 | NP_495146 | 1 | 218 | 218 | K05F1.9 | medium | high | not |
B | 1 | NP_495062 | 1 | 210 | 210 | Helix Loop Helix family member (hlh-26) | high | high | not |
B | 2 | NP_496422 | 1 | 225 | 225 | B0491.3 | high | low | Yes |
B | 3 | NP_495475 | 1 | 197 | 240 | F10E7.2 | not soluble | low | not |
B | 4 | NP_496547 | 21 | 284 | 284 | W03C9.1 | medium | high | not |
B | 5 | NP_496156 | 29 | 184 | 184 | R53.8 | low | high | not |
B | 8 | NP_501161 | 1 | 250 | 250 | F42C5.3 | medium | high | Yes |
B | 9 | NP_502163 | 1 | 319 | 319 | C10C6.3 | high | high | Yes |
B | 10 | NP_500772 | 55 | 348 | 368 | ZK354.6 | not soluble | not soluble | not |
B | 11 | NP_501789 | 1 | 297 | 297 | F25H8.1 | high | high | Yes |
C | 1 | NP_501895 | 1 | 294 | 294 | R09E10.1 | medium | high | Yes |
C | 2 | NP_500890 | 1 | 243 | 243 | H32C10.2 | high | high | not |
C | 3 | NP_501981 | 1 | 388 | 388 | R102.5a | low | low | not |
C | 4 | NP_507039 | 1 | 196 | 196 | F14H3.5 | high | low | not |
C | 5 | NP_506094 | 1 | 183 | 183 | F23H12.3 | medium | low | not |
C | 6 | NP_506094 | 1 | 90 | 183 | F23H12.3 | not soluble | medium | Yes |
C | 8 | NP_505964 | 20 | 260 | 260 | T04F3.2 | medium | medium | not |
C | 9 | NP_506495 | 1 | 252 | 252 | D1086.4 | high | high | not |
C | 10 | NP_741113 | 1 | 394 | 419 | C32A3.3a | not soluble | not soluble | not |
C | 11 | NP_501199 | 1 | 299 | 299 | F55G1.9 | not soluble | low | not |
D | 1 | NP_495021 | 1 | 197 | 197 | EEED8.12 | high | high | not |
D | 6 | NP_502315 | 1 | 199 | 199 | F35G2.2 | high | high | Yes |
D | 10 | NP_491869 | 1 | 232 | 232 | MeDiaTor family member (mdt-18) | medium | low | not |
E | 1 | NP_501936 | 1 | 190 | 190 | F01D4.5b | medium | low | not |
E | 2 | NP_506929 | 26 | 144 | 206 | F57A10.4 | not soluble | low | not |
E | 3 | NP_506929 | 26 | 206 | 206 | F57A10.4 | not soluble | not soluble | not |
E | 5 | NP_491210 | 1 | 249 | 249 | T12F5.1 | high | high | not |
E | 7 | NP_506245 | 35 | 240 | 240 | R186.3 | low | not soluble | not |
E | 8 | NP_495941 | 1 | 269 | 308 | T24H10.1 | high | medium | Yes |
E | 10 | NP_510298 | 1 | 269 | 269 | AMP-Activated Kinase Beta subunit family member (aakb-1) | medium | low | not |
F | 1 | NP_492285 | 1 | 239 | 239 | F02E9.5 | medium | low | not |
F | 2 | NP_509787 | 1 | 195 | 195 | F13E6.1 | medium | high | not |
F | 3 | AAZ82857 | 1 | 230 | 230 | Hypothetical protein C17H12.13 | not soluble | not soluble | not |
F | 4 | NP_493382 | 1 | 210 | 210 | Y87G2A.10 | high | medium | Yes |
F | 5 | NP_497990 | 1 | 214 | 214 | C38D4.9 | high | high | Yes |
F | 10 | NP_498391 | 1 | 217 | 217 | C56G2.15 | high | high | Yes |
G | 2 | NP_493230 | 1 | 183 | 183 | W02A11.2 | high | not soluble | Yes |
G | 3 | NP_492005 | 1 | 189 | 189 | F22D6.2 | high | high | Yes |
G | 4 | NP_492692 | 1 | 206 | 206 | Y106G6E.4 | high | medium | Yes |
G | 5 | NP_492795 | 1 | 207 | 207 | C34B2.5 | high | medium | Yes |
G | 6 | NP_491736 | 1 | 214 | 214 | C06A5.2 | medium | high | not |
G | 7 | NP_491358 | 1 | 233 | 233 | ZK973.9 | low | medium | not |
G | 8 | NP_492301 | 1 | 240 | 240 | D1081.9 | not soluble | not soluble | not |
G | 9 | NP_492301 | 1 | 65 | 240 | D1081.9 | high | high | Yes |
G | 11 | NP_491721 | 1 | 273 | 273 | B0207.11 | high | high | not |
H | 2 | NP_491965 | 1 | 274 | 274 | T21G5.4 | high | low | Yes |
H | 3 | NP_491348 | 1 | 287 | 287 | Y47D9A.2a | not soluble | not soluble | not |
H | 4 | NP_491903 | 27 | 330 | 363 | D2092.4 | high | medium | not |
H | 5 | NP_496803 | 1 | 183 | 183 | F15D4.2 | medium | low | not |
H | 6 | NP_491434 | 1 | 177 | 177 | C10H11.7 | low | low | not |
H | 8 | NP_495527 | 30 | 179 | 179 | F45E12.5b | high | high | Yes |
H | 9 | NP_494315 | 1 | 276 | 276 | F22E5.8 | not soluble | not soluble | not |