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Table 2 Comparisons between experimental and DDBP prediction results*

From: Domain selection combined with improved cloning strategy for high throughput expression of higher eukaryotic proteins

A

 

B

C

D

E

F

G

H

I

J

K

I

11058-C7

249

no

no

4–190; 1–220; 80–190

no

24%, (7–240/8–268, 288); 26%, (4–240/7–225, 251)

(1–249)#

1–249

NP_506406

F20G2.1

I

11048-D3

199

no

no

9–199

76–98, 181–181

26%, (5–160/3–164, 208)

(1–199)$

1–199

NP_502315

F35G2.2

I

11011-D8

190

no

no

8–36, 47–75, 83–111; 2–107;

148–172, 108–120, 82–92, 49–51

27%, (37–105/1–69, 146)

(45–190)&

1–107, 52–190

NP_493641

F23F1.2

I

18-A2

210

no

no

29–79; 108–194

no

30%, (75–193/4–115, 135)

(74–210)&

75–210

NP_491893

BAG1 (human) homolog family member (bag-1)

I

11033-F3

208

no

no

6–74; 128–194; 1–97; 80–207

39–56

31%, (2–207/1–197, 198)

(1–208)#

1–208

NP_496863

Glutathione S-Transferase family member (gst-16)

I

11-D11

346

no

no

80–317; 55–320; 219–317

19–34, 116–126

31%, (76–334/36–291, 298)

(56–346)&

55–346

NP_491872

C55B7.3

I

11104-F4

370

no

no

2–221, 1–370

126–143, 348–352, 19–25, 71–82, 292–297, 233–237

34%, (128–347/15–231, 265)

(125–370, 1–124)&

1–125, 128–347

NP_001040820

Cell Division Cycle related family member (cdc-37)

I

79-D4

401

no

no

65–395; 37–400

66–108, 27–45, 108–125, 217–236, 138–146

35%, (212–395/2– 185, 185)

(206–401)#

212–401

NP_491735

C06A5.7b

I

9-H3

212

no

no

no

19–52, 162–192, 79–89

35%, (86–136/84– 131, 217)

(59–212)$

53–212

NP_493365

Y40B1B.5

I

76-D4

254

no

no

2–171; 3–250; 139–167

139–147

36%, (3–251/8–265, 278)

(1–254)#

1–254

NP_001021765

Y47G6A.22

I

8-C1

142

no

no

4–140

no

46%, (5–141/9–149, 150)

(1–142)&

1–142

NP_499813

T12D8.6

I

11-F6

327

no

209– 231, 246– 268

207–227, 246–266

56–95, 19–56, 95–136, 136–161

50%, (141–167/1– 28, 163)

(1–182, 1–145)&

1–135

NP_491774

T09B4.5a

I

1-F11

229

no

no

148–217; 170–201; 170–212

19–21, 129–134

59%, (135–220/20–107, 113)

(135–229)#

135–229

NP_506367

F53F4.3

I

3-H6

431

no

no

24–118, 141–234; 254–370; 261–370

397–413, 214–225, 100–107, 123–128

60%, (4–131/20–147, 149); 48%, (4–244/41–280, 280); 40%, (7–408/24–422, 457); 35%, (128–428/22–330, 336);

(1–135)#

1–131, 128–244, 245–431

NP_508026

FK506-Binding protein family member (fkb-6)

I

20-H6

496

no

no

38–496; 186–261, 293–363, 422–483; 183–272, 275–374, 422–487

120–151, 265–279

66%, (394–496/1– 103, 104) ; 75%, (183–269/1– 87, 90); 75%, (290–366/1– 78, 85)

(169–385, 386–496)&

183–272, 290–366, 394–496

NP_001022967

U2AF splicing factor family member (uaf-1)

I

1-D10

206

1–21

no

41–198; 23–77, 85–141, 143–196

no

no

(23–206)#

22–206

NP_491320

R12E2.13

I

11020-H6**

494

no

no

53–225; 337–475; 336–453; 236–494

19–52, 106–145, 215–241, 373–383, 325–330

no

(1–237, 238–494) &$

53–225, 236–494, 331–494

NP_493412

Y37H9A.3

I

70-H8

130

no

107– 129

109–129

19–104

no

(1–130)#

1–130

NP_491052

W03D8.3

I

8-C9

183

no

no

125–159;

no

no

(1–183)# ; (28–183, 23–183)$

1–183

NP_510277

BMP receptor Associated protein family member (bra-1)

I

11005-B8

245

no

no

no

19–20, 129–136, 41–46

no

(9–245)#

1–245

NP_740981

R05F9.1b

II

18-F7

288

no

no

34–286

19–30, 72–78, 196–202, 66–70

45%, (35–286/21– 274, 276)

(32–266)$

34–286

NP_001021584

EXOnuclease family member (exo-3)

II

4-F5

592

no

no

189–269

485–509, 125–142, 19–37, 369–382,99–109, 311–317

35%, (210–269/15–74, 76);

(1–144)&

1–124, 143–269

NP_494544

C16C8.16

II

11011-C6

162

no

no

4–162; 5–54, 71–132

51–68

no

(1–148, 1–124)&

1–162

NP_500324

F42A6.6

II

11058-H2

249

no

no

4–190; 4–230; 4–209

no

40%, (5–245/23– 263, 267)

(1–222)$

1–249

NP_506407

F20G2.2

II

76-F10

263

no

no

23–130; 33–109

118–126, 20–20

27%, (27–120/6–98, 108)

(1–129)&

21–130

T26031

hypothetical protein W01A8.2

II

79-H11

245

no

no

2–156, 1–241

144–181, 181–204, 121–144

51%, (2–154/2–154, 155)

(1–185)$

1–156

NP_492567

C03D6.5

II

25–B11

302

no

no

23–127; 45–114

203–236, 19–24, 236–248, 146–161, 187–195

no

(1–153)&

23–145

NP_492781

B0511.7

II

11-D3

313

no

no

28–214; 9–302

151–174, 19–22

no

(1–313)# ; (213–313)$

23–313

NP_001021333

Suppressor of PResenilin defect family member (spr-2)

II

11058-F12

272

no

no

40–63; 40–68, 90–124

no

32%, (67–119/3–54, 60); 26%, (40–119/5–84, 87); 31%, (41–114/36– 113, 124)

(1–147)&

1–124

NP_503566

F36F12.8

III

20-D7

500

no

no

311–395; 41–278; 283–436

387–417, 288–308, 453–469, 481–482

28%, (342–432/68–153, 289)

(1–500)# ; (298–500, 388–500, 407–500)$

1–287, 283–452

NP_491868

lariat DeBRanching enzyme related family member (dbr-1)

III

37-G9

245

no

no

no

206–227, 19–21

no

(1–102, 103–245)&

1–245

NP_507040

F14H3.6

III

70-D2

265

1–25

15–37

69–243

223–247, 195–199

22%, (128–264/13–121, 135)

(1–130, 1–174)&

1–265

AAC25860

Hypothetical protein C37C3.3

III

2-B6

316

no

no

27–294

225–260, 174–191, 149–163, 296–298, 71–82, 84–84, 219–220

23%, (53–168/46– 180, 201)

(71–294)# ; (104–316)$&

1–295

NP_501422

D2096.8

III

10-E5

274

no

no

1–80, 108–190

229–246, 193–213

25%, (17–161/32– 166, 196)

(65–237, 1–74, 75–274)&

1–192

NP_502380

C25G4.6

III

3-D2

419

no

no

95–128, 133–166; 93–197; 133–166

196–217, 41–62, 259–275, 341–351, 19–21

26%, (95–239/13– 144, 166); 32%, (99–197/8–95, 118)

(140–309, 290–419)&

93–197, 93–258

NP_495087

C17G10.2

III

113-H8

588

no

no

232–342; 241–334

492–515, 19–56, 81–132, 150–185

29%, (234–328/2– 85, 105)

(1–345, 34–313)&

232–342

NP_740981

R05F9.1b

III

76-F6

803

no

769– 791

17–250, 558–652; 10–34, 111–148, 382–402

752–767, 19–24, 717–727, 280–281

29%, (62–257/5– 200, 205); 29%, (83–257/1– 175, 181)

(1–181)&

25–257

NP_491008

alpha-CaTuliN (catenin/vinculin related) family member (ctn-1)

III

4-A4

569

no

no

164–338, 369–527;187–200, 206–222, 263–279, 305–321, 321–335, 506–527; 263–318, 388–452, 511–568

65–97, 19–38,112–139

32%, (154–567/18–397, 402)

(334–542, 334–501)&; (1–569)#

154–549

NP_495753

associated with RAN (nuclear import/export) function family member (ran-3)

III

25-H8

339

no

no

24–84; 24–75

215–276, 111–169, 187–201, 100–109

41%, (22–75/7–61, 70)

(1–152)&

1–99

NP_495652

T09A5.8

III

2-H9

356

no

no

22–273; 1–108, 115–297; 13–284

192–215, 303–312, 338

42%, (1–284/1–289, 382)

(1–335, 1–356) $&

1–197

NP_497949

T23F11.1

III

18-H1

208

no

no

32–124; 36–113; 24–45, 51–68, 131–145, 164–181, 186–205

171–190, 114–171, 19–40

43%, (41–110/10– 76, 90)

(1–81)&$

32–124

NP_510410

HIStone family member (his-24)

III

11049-D6

435

no

no

293–433; 270–433

110–152, 234–260, 175–186, 375–381

no

(1–156)# ; (9–158, 1–119)$&

261–435

NP_001041025

Y41E3.7a

III

9-G11

250

no

no

1–194

228–232

no

(35–250)&

1–227

NP_497076

R05H10.1

III

10-E1

251

no

no

no

158–199

no

(1–206)&

1–251

NP_496943

W01G7.4

III

37-F11

230

no

no

1–230

106–126, 168–182

no

(45–179, 45–230)&

1–230

NP_507024

T10C6.5

III

75-A8

228

no

no

no

19–44, 125–135

no

(1–189)$&

1–228

NP_492509

F46A9.1

III

11048-E2

262

no

no

33–178; 61–179

183–198

no

(1–262)$

1–182

NP_501337

MEChanosensory abnormality family member (mec-17)

  1. * column A: Prediction accuracy level (I, accurate; II, basically accurate; III, wrong) and Plate-ID, users could query/search the sequence relative information from the SGCE web site [41]; column B: number of amino acids in the ORF; column C: signal peptide prediction results using SignalP; column D: transmembrane region prediction results using TMHMM; column E: domains/fragments from Interpro/InterProScan analysis; column F: domain linker regions predicted by Domain Linker Finder; column G: PDB-alignment results, including the percentage of sequence identity, query/subject sequence start position and end position, and the length of the subject sequence; column H: experimental results from protein crystal/three-dimensional structures (labeled with #), limited proteolysis (labeled with $) or spontaneous degradation (labeled with &); column I: DDBP prediction results; J: Accession Number, user could obtain sequence relative information from National Center for Biotechnology Information (NCBI) [40]; K: Definition of the protein or ACEID for proteins without known functions.
  2. ** 11020-H6 is corresponding to the region 299–792 of NP_493412.