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Figure 6 | BMC Biotechnology

Figure 6

From: Domain selection combined with improved cloning strategy for high throughput expression of higher eukaryotic proteins

Figure 6

Soluble expression results of 57 proteins used for testing DDBP method. ELISA results for soluble expression at 18°C and 37°C. Different shades in panels stand for different expression levels: the dark gray for the higher level, the gray for the medium level, the white for the lower level and the black for those not expressed, which was decided by comparisons with the positive control (A12 and B12, each containing one soluble protein). If ELISA readings of OD (optical density) at 405 nm was higher than or the same with the lower value of positive controls, the protein in this well was considered as expressed. Well C12 and D12 are negative controls and blank wells (white with no numbers) are null. After comparing the results at 18°C and 37°C, seven proteins (well B10, C10, E3, F3, G8, H3, and H9) were considered as not soluble.

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