Knockdown of the bovine prion gene PRNP by RNA interference (RNAi) technology

BMC Biotechnology

Table 2 Target sequences examined

No.	Position^a	sequence	nt	Constructed vectors (○)^b				Value^c
				hU6	CACC-S/K	S/K	tRNA
1	132–150	GGGCAGTCCTGGAGGCAAC	19	○	○	○	○	0.779
2	132–153	GGGCAGTCCTGGAGGCAACCGT	22	○	○	○	○
3	372–390	AGGAGCTGCTGCAGCTGGA	19	○	○	○	○	0.775
4	372–393	AGGAGCTGCTGCAGCTGGAGCA	22	○	○	○	○
5	569–591	GTGTCAATATCACAGTCAAGGA	22	○				0.757
6	616–634	GGGGAGAACTTCACCGAAA	19	○	○	○	○	0.852
7	616–635	GGGGAGAACTTCACCGAAAC	20	○	○	○	○
8	616–636	GGGGAGAACTTCACCGAAACT	21	○	○	○	○
9	616–637	GGGGAGAACTTCACCGAAACTG	22	○	○	○	○
10	616–634	GGGGAGAACTTCACCGAAACTGA	23	○	○	○	○
11	616–634	GGGGAGAACTTCACCGAAACTGAT	24	○
12	616–634	GGGGAGAACTTCACCGAAACTGACA	25	○	○	○	○
13	616–634	GGGGAGAACTTCACCGAAACTGACATC	27	○	○	○	○
14	616–634	GGGGAGAACTTCACCGAAACTGACATCAA	29	○	○	○	○
15	725–743	GTGTGATCCTCTTCTCTTC	19	○	○	○	○	0.772
16	725–746	GTGTGATCCTCTTCTCTTCCCC	22	○	○	○	○
17	730–748	ATCCTCTTCTCTTCCCCTC	19	○				0.752
18	730–751	ATCCTCTTCTCTTCCCCTCCTG	22	○

a, Position 1 starts from A of ATG, the first codon of bPRNP. b, hU6, CC-S/K, S/K, and tRNA indicate piGENE hU6, piGENE CACC-S/K, piGENE S/K, and piGENE tRNA, respectively (Table 3). Open circles designate construction of vectors. c, Values were obtained from the prediction algorithm [20].

ISSN: 1472-6750