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Table 1 Characteristics of target vectors

From: Knockdown of the bovine prion gene PRNP by RNA interference (RNAi) technology

Vector Promoter 1 Reporter 1 Target Promoter 2 Reporter 2 Target
pEGFP-bPrP CMV EGFP bPRNP    
pGL3-bPrP SV40 Fire-fly luciferase bPRNP    
pFluc-bPrP-Rluc SV40 Fire-fly luciferase bPRNP HSV-TK Renilla luciferase*  
pFluc-Rluc-bPrP SV40 Fire-fly luciferase*   HSV-TK Renilla luciferase bPRNP
pTKFluc-bPrP-Rluc HSV-TK Fire fly luciferase bPRNP HSV-TK Renilla luciferase*  
  1. *, used as the internal control.