Table 1 Characteristics of target vectors
From: Knockdown of the bovine prion gene PRNP by RNA interference (RNAi) technology
Vector | Promoter 1 | Reporter 1 | Target | Promoter 2 | Reporter 2 | Target |
|---|---|---|---|---|---|---|
pEGFP-bPrP | CMV | EGFP | bPRNP | Â | Â | Â |
pGL3-bPrP | SV40 | Fire-fly luciferase | bPRNP | Â | Â | Â |
pFluc-bPrP-Rluc | SV40 | Fire-fly luciferase | bPRNP | HSV-TK | Renilla luciferase* | Â |
pFluc-Rluc-bPrP | SV40 | Fire-fly luciferase* | Â | HSV-TK | Renilla luciferase | bPRNP |
pTKFluc-bPrP-Rluc | HSV-TK | Fire fly luciferase | bPRNP | HSV-TK | Renilla luciferase* | Â |