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Figure 2 | BMC Biotechnology

Figure 2

From: Knockdown of the bovine prion gene PRNP by RNA interference (RNAi) technology

Figure 2

Silencing of b PRNP by siRNA as revealed by immunostaining. HeLaS3 cells were co-transfected with pbPrP- FLAG DNA and the control vector DNA of piGENE tRNA (A) or siRNA expression vector DNA of piGENE tRNA-616-21, the sequence of which is No. 8 in Table 2 (B). Cells were stained with SAF32 antibody. HeLaS3 cells were co-transfected with pbPrP- FLAG DNA and the control vector DNA of piGENE hU6 (C) or siRNA expression vector DNA of piGENE hU6-616-21(D). Cells were stained with P6488 antibody. HeLaS3 cells were co-transfected with pshort-bPrP- FLAG DNA and the control vector DNA of piGENE hU6 (E) or siRNA expression vector DNA of piGENE hU6-616-21 (F). Cells were stained with P6488 antibody.

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