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Figure 5 | BMC Biotechnology

Figure 5

From: Lentiviral vectors encoding tetracycline-dependent repressors and transactivators for reversible knockdown of gene expression: a comparative study

Figure 5

Time course of CXCR4 knockdown. (A) HOS-CD4-Fusin cells were co-transduced with lentiviral vectors as described in Figure 4. Transduced cells were incubated in the absence of Dox. On day 6, Dox was added to the culture medium, and cells were analyzed for CXCR4 receptor levels by flow cytometry at various times. (B) HOS-CD4-Fusin cells were co-transduced with lentiviral vectors as described in Figure 4. Cells were incubated in the presence of Dox for 6 days. Dox in the culture medium was then removed and cells were analyzed for CXCR4 receptor levels by flow cytometry at various times.

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