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Figure 3 | BMC Biotechnology

Figure 3

From: Plastoglobules: a new address for targeting recombinant proteins in the chloroplast

Figure 3

Expression of PGL34 g -YFP in planta. A. Schematic representation of the construct used for plant transformation. The coding sequence of the yellow fluorescent protein (YFP) was inserted in the genomic sequence of PGL34, replacing the PGL34 stop codon. Boxes indicate coding regions. B. Western blot analysis of rosette leaves from homozygous T2 transgenic lines. PGL34-YFP was detected with anti-GFP antibodies. Proteins were visualized on the membrane by staining with amidoblack. Wt, wild type Arabidopsis plant protein extract. C. Yield estimation. Different amounts of total protein extracts from line 5.2 were subjected to SDS-PAGE in parallel with defined amounts of recombinant GST-GFP and chemiluminescent immunoblot signals were quantified. D. Detection of PGL34-YFP by fluorescence microscopy. YFP fluorescence was detected in leaves, cotyledons and roots by illuminating the seedlings with UV light. Scale bars: 0.5 mm. E. Subcellular localization of PGL34-YFP in leaves was determined using confocal laser scanning microscopy. Scale bars: 10 μm.

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