Figure 4

Identification of cell populations simultaneously expressing GFPVP2 and VP6. Cultures shown in panels A to C were used for setting the gating limits for analysis of coinfected cultures. A. Uninfected control culture. B. Culture at 24 hpi infected with bacGFPVP2 at a MOI of 5 pfu/cell. C. Culture at 24 hpi infected with bacVP6 at a MOI of 5 pfu/cell. D. Culture coinfected with bacGFPVP2 and bacVP6 with a MOI of 0.1 pfu/cell of each virus, 24 hpi. E. Culture coinfected with bacGFPVP2 and bacVP6 with a MOI of 0.1 pfu/cell of each virus, 48 hpi.