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Figure 1 | BMC Biotechnology

Figure 1

From: Cloning and characterization of an adenoviral vector for highly efficient and doxycycline – suppressible expression of bioactive human single – chain interleukin 12 in colon cancer

Figure 1

Vector construction. Construction of recombinant adenoviral vectors expressing human IL12 under control of doxycycline by calcium phosphate-mediated coprecipitation of the corresponding plasmids in 293 cells. VP16: Herpes simplex virus transactivator gene, TetR: Tetracycline repressor gene, TKmin: Thymidine kinase minimal promoter, TetO7: Tetracycline operator (seven copies), CMVmin: Cytomegalovirus minimal promoter, hscIL-12: human single-chain IL-12 gene, Amp R: ampicillin resistance gene, ITR: inverted terminal repeat, pA: poly-A from the bovine growth hormone gene.

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BMC Biotechnology

ISSN: 1472-6750

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