Table 2 Preparative isolation and purification of AOX from the mutant strains (on the example of CA2).
Stage | Result | Specific AOX activity, μmol·min-1·mg-1 of protein (U·mg-1) | Total AOX yield, U (%)* |
|---|---|---|---|
Cell growth | 17.7 g of cells | 0.35 (per 1 mg of cells) | 6130 (100%) |
Cell disintegration | Crude extract, mg 2300 | 2.0 | 4600 (75%) |
Fractionation by ammonium sulphate (30 – 70%) | Protein precipitate (70% of ammonium sulphate saturation), mg 750 | 4.0 | 3000 (49%) |
Chromatography on DEAE – Toyopearl 650 M | Protein sample, mg 75 | 16.0 | 1200 (20%) |