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Table 2 Preparative isolation and purification of AOX from the mutant strains (on the example of CA2).

From: Isolation and characterization of mutated alcohol oxidases from the yeast Hansenula polymorpha with decreased affinity toward substrates and their use as selective elements of an amperometric biosensor

Stage Result Specific AOX activity, μmol·min-1·mg-1 of protein (U·mg-1) Total AOX yield, U (%)*
Cell growth 17.7 g of cells 0.35 (per 1 mg of cells) 6130 (100%)
Cell disintegration Crude extract, mg 2300 2.0 4600 (75%)
Fractionation by ammonium sulphate (30 – 70%) Protein precipitate (70% of ammonium sulphate saturation), mg 750 4.0 3000 (49%)
Chromatography on DEAE – Toyopearl 650 M Protein sample, mg 75 16.0 1200 (20%)
  1. * 1 Unit is equal 1 μmol of converted substrate per 1 min at standard conditions of assay (20°C).