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Table 2 Effects of IbpAB co-overproduction (combinations 6 to 8) on the yields of purified, soluble target proteins.

From: Chaperone-based procedure to increase yields of soluble recombinant proteins produced in E. coli

Protein

MW (kDa)

Increased solubility by

  

one step procedure

two step procedure

  

Yield increase

Best chaperone combination

Yield increase

Best chaperone combination

Ag1Ser

30

∞

7

3

7

Chip

87

∞

8

4

8

dLMO

92

∞

6

3

6

Isu2

46

∞

6

3

8

Kringle

27

∞

8

7

8

Luc7

56

∞

8

4

8

Oskar1

16

∞

8

3

8

Ag3Ser

33

∞

6

1

-

Ag4Ser

35

∞

8

1

-

Icy1

45

∞

8

1

-

Oskar4

70

∞

6

1

-

2c18

50

2.5

7

3.5

6

E8R1

85

2.5

6

3

7

Oskar2

115

1*

-

∞

6

Ag2Ser

31

2.5

7

1

-

Synapsin

62

3

6

1

-

1Tep3

47

18

6

1

-

1Tep4

45

6

8

1

-

3x77

44

1

-

3

8

Titin

35

1

-

4

7

Xklp3A3

72

1

-

1

-

Msl1

66

1

-

1

-

NF1

36

1*

-

1*

-

  1. Increases in the amount of purified, soluble protein are given relative to the optimal solubility (set as 1) obtained either by the best of the combinations 1 to 6 tested before (Table 1) or to control cells (combination 4, data not shown). Symbols as in Table 1.
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BMC Biotechnology

ISSN: 1472-6750

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