Skip to main content

Table 2 Effects of IbpAB co-overproduction (combinations 6 to 8) on the yields of purified, soluble target proteins.

From: Chaperone-based procedure to increase yields of soluble recombinant proteins produced in E. coli

Protein MW (kDa) Increased solubility by
   one step procedure two step procedure
   Yield increase Best chaperone combination Yield increase Best chaperone combination
Ag1Ser 30 7 3 7
Chip 87 8 4 8
dLMO 92 6 3 6
Isu2 46 6 3 8
Kringle 27 8 7 8
Luc7 56 8 4 8
Oskar1 16 8 3 8
Ag3Ser 33 6 1 -
Ag4Ser 35 8 1 -
Icy1 45 8 1 -
Oskar4 70 6 1 -
2c18 50 2.5 7 3.5 6
E8R1 85 2.5 6 3 7
Oskar2 115 1* - 6
Ag2Ser 31 2.5 7 1 -
Synapsin 62 3 6 1 -
1Tep3 47 18 6 1 -
1Tep4 45 6 8 1 -
3x77 44 1 - 3 8
Titin 35 1 - 4 7
Xklp3A3 72 1 - 1 -
Msl1 66 1 - 1 -
NF1 36 1* - 1* -
  1. Increases in the amount of purified, soluble protein are given relative to the optimal solubility (set as 1) obtained either by the best of the combinations 1 to 6 tested before (Table 1) or to control cells (combination 4, data not shown). Symbols as in Table 1.