Table 1 List of proteins co-expressed without and with chaperone combinations 1 to 5 and effects of chaperones on yields of purified, soluble target proteins, applying either the one-step or two-step procedure.

43

∞

4

3

4

54

∞

4

3

3

52

∞

3

3

4

41

∞

1

n.d.

n.d.

118

∞

2/4

2.5

2

50

3

4

8

4

68

3.5

4

13

4

55

7

3

42

4

29

3

4

7

5

8

3

5

6

1

86

2.5

3

5

5

20+ 16

2.5

4

3.5

4

59

3.5

3

3.5

3

85

5.5

3

5.5

3

35

3

4

3

4

9

3.5

4

3.5

4

43

4.5

3

4.5

3

44

2.5

5

2.5

5

70

2.5

4

2.5

4

90+ 86

3

4

3

4

47

4

3

n.d.

n.d.

45

3.5

5

n.d.

n.d.

64

3

5

n.d.

n.d.

58

5

5

n.d.

n.d.

7.5

3

4

n.d.

n.d.

30

3.5

3

n.d.

n.d.

62

1*

-

∞

2

40

1*

-

∞

5

55

1

-

2

4

35

1

-

19

4

70

1

-

11

2

55

1

-

28

4

91

1

-

3

2

22

1

-

3

4

95

1

-

1

-

72

1

-

1

-

11

1

-

1

-

52

1

-

n.d.

n.d.

64

1

-

n.d.

n.d.

37

1

-

n.d.

n.d.

38

1

-

n.d.

n.d.

42

1*

-

1*

-

30

1*

-

1*

-

37

1*

-

1*

-

33

1*

-

1*

-

57

1*

-

n.d.

n.d.

38

1*

-

n.d.

n.d.

72

1*

-

n.d.

n.d.

35

1*

-

n.d.

n.d.

108

1*

-

n.d.

n.d.