Skip to main content

Table 1 List of proteins co-expressed without and with chaperone combinations 1 to 5 and effects of chaperones on yields of purified, soluble target proteins, applying either the one-step or two-step procedure.

From: Chaperone-based procedure to increase yields of soluble recombinant proteins produced in E. coli

Protein MW (kDa) Increased solubility by
   one step procedure two step procedure
   Yield increase Best chaperone combination Yield increase Best chaperone combination
1Fringe 43 4 3 4
2Ap 54 4 3 3
22f21 52 3 3 4
LZip 41 1 n.d. n.d.
Oskar3 118 2/4 2.5 2
2c18 50 3 4 8 4
2Tep4 68 3.5 4 13 4
BtKe 55 7 3 42 4
PhK 29 3 4 7 5
Tep1 8 3 5 6 1
Susy 86 2.5 3 5 5
Xklp3A+Xklp3B 20+ 16 2.5 4 3.5 4
B1R 59 3.5 3 3.5 3
E8R2 85 5.5 3 5.5 3
GTR1 35 3 4 3 4
HbpH 9 3.5 4 3.5 4
Rolled 43 4.5 3 4.5 3
Xklp3A4 44 2.5 5 2.5 5
Xklp3B3 70 2.5 4 2.5 4
Mash+ Susy 90+ 86 3 4 3 4
1Tep3 47 4 3 n.d. n.d.
1Tep4 45 3.5 5 n.d. n.d.
BtKc 64 3 5 n.d. n.d.
E8R1 58 5 5 n.d. n.d.
MaxF 7.5 3 4 n.d. n.d.
Tev prot. 30 3.5 3 n.d. n.d.
Xklp3A1 62 1* - 2
Xklp3B1 40 1* - 5
2Fringe 55 1 - 2 4
Xklp3A5 35 1 - 19 4
2Tep3 70 1 - 11 2
BtKp 55 1 - 28 4
Mash 91 1 - 3 2
PPAT 22 1 - 3 4
Eg5 95 1 - 1 -
F10L 72 1 - 1 -
Tep2 11 1 - 1 -
1AP 52 1 - n.d. n.d.
Chip 64 1 - n.d. n.d.
dLMO 37 1 - n.d. n.d.
Pex5P 38 1 - n.d. n.d.
Endostatin 42 1* - 1* -
Kringle 30 1* - 1* -
Lzip2 37 1* - 1* -
UCP1 33 1* - 1* -
Tlc4 57 1* - n.d. n.d.
Xklp3A2 38 1* - n.d. n.d.
Xklp3A3 72 1* - n.d. n.d.
Xklp3B1 35 1* - n.d. n.d.
Xpot1 108 1* - n.d. n.d.
  1. The increase in solubility of target proteins relative to control cells without co-overexpression of chaperones (set as 1) is given in the column "yield increase". The chaperone combinations for optimal yield of each target protein in the one-step and two-step procedures are indicated. Proteins that exhibited solubility exclusively after chaperone co-overexpression are indicated by "∞". The symbol "*" indicates that no soluble protein was purified from cells with or without chaperone co-overproduction. n.d., not determined. The same protein name can appear more than one time in the list because different constructs (domains or fusions) have been used for its expression.