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Figure 4 | BMC Biotechnology

Figure 4

From: Recombinant Escherichia coli produces tailor-made biopolyester granules for applications in fluorescence activated cell sorting: functional display of the mouse interleukin-2 and myelin oligodendrocyte glycoprotein

Figure 4

Enterokinase treatment of antigen displaying PHA granules and FACS analysis. PhaP-IL2 and PhaP-MOG granules were incubated with enterokinase for the indicated period of time, washed and then tested for the amount of native proteins remaining at the surface of the granules. In (A), the anti-IL2 PC61 and anti-MOG 8-18C5 monoclonal antibodies were used for this purpose as described for Figure 1. In (B), the percentage of IL2 protein detected after 1 and 16 hours incubation with enterokinase is shown. The data was depicted in a "normalized" fashion (% of Max). One representative experiment of at least three performed experiments, which showed no significant differences, is depicted. After overnight incubation no detectable staining using fluorescent anti-IL2 antibodies could be observed.

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BMC Biotechnology

ISSN: 1472-6750

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