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Figure 3 | BMC Biotechnology

Figure 3

From: Recombinant Escherichia coli produces tailor-made biopolyester granules for applications in fluorescence activated cell sorting: functional display of the mouse interleukin-2 and myelin oligodendrocyte glycoprotein

Figure 3

PHA granules display the native eukaryotic proteins. PhaP-IL2 and PhaP-MOG granules were produced as described in Materials and Methods. Approximately 5 × 108 PhaP-IL2 or PhaP-MOG granules were incubated with PC61 (anti-IL2) or 8-18C5 (anti-MOG) antibodies. Samples were collected using a FACsCalibur and then analyzed on FlowJo software. The data were depicted in a "normalized" fashion (% of Maximum). The % of Max is the number of cells in each bin divided by the number of cells in the bin that contains the largest number of cells. One representative experiment of at least three independent experiments, which showed no significant differences, is shown.

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BMC Biotechnology

ISSN: 1472-6750

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