Physiological evaluation of the filamentous fungus Trichoderma reesei in production processes by marker gene expression analysis

Rautio, Jari J; Bailey, Michael; Kivioja, Teemu; Söderlund, Hans; Penttilä, Merja; Saloheimo, Markku

doi:10.1186/1472-6750-7-28

Table 1 T.reesei genes used in the TRAC analysis grouped into functional categories.

From: Physiological evaluation of the filamentous fungus Trichoderma reesei in production processes by marker gene expression analysis

Gene name	Function	Probe Length (nt)	Probe sequence 5'-3'	Location of the probe in CDS	Reference data
Carbohydrate degradation/oxidation
bgl2	β- glucosidase	41	CGTTATAGTACTTGACCCTGAAGTCATCTTCGAGAATCTTC	1143–1183	51
cbh1	Cellobiohydrolyase	25	CATTCTGGACATAGTATCGGTTGAT	889–913	19
egl1	Endoglucanase	27	CGGACTTTGTACACTTGTAGGTTGTCA	107–133	19
lac1	Melanocarpus albomyces laccase	35	GACCAACGCATGTCGAAAGTGAACAACAAGTAACC	1952–1986	24
Stress
bip1	Protein chaperon	27	AGGGGGTTGACGTCCATGAGAACAATG	1008–1034	58
gcn4	Transcription factor	37	TGAAGAAGACGATCGGTACATGGGCTCTGATTCCAAA	347–410	52
hac1	Transcription factor	31	AGAGAGTGATGCTGTCCTGGAGAGAGTCGAG	562–592	58
hsp105	ER chaperon	37	CGGGCTTATCCTCAGTGTCAACTTGTTGATAGAATAA	1522–1558	37
hsp30	Heat shock protein 30	31	GTACTTTGCGTTGTCGGTAGGCTTGTTGCTG	750–780	36
msn4	Transcription factor	35	CGAGAGAACTTCTTGCCGCACTCGTTGCACTCAAA	1327–1361	5
nsf1	General membrane fusion factor	41	CAACAGGGCATCGTCAATCATGTCTTTTCGATTCGTCATTC	1388–1428	59
nth1	Neutral trehalase	43	AACGTAACTGGCATTGACCCATCCAAATCCTTCTTTCGCAACG	2061–2103	30
pdi1	Protein disulfide isomerase	33	GGTCAAAGGGGAACTTGAGGTTCTTCTCAATGT	926–958	58
sod2	Superoxide dismutase	29	TTGATGACGTCCCAGATGGCGCTGAAGTA	637–665	5
tps1	Trehalose-6-P-phosphate synthase	41	AACTTGCGGATGAACTTGGTGATCCACGACTGGACATTCTG	1612–1652	53
trr1	Thioredoxin reductase	31	AATGACGAAGAGGGGCTTGTTGCGGAAGATG	444–474	18
trx2	Thioredoxin protein	39	CAAACTTGACAAAGTGGACCTTGTCCTTGAACTCTGCGT	230–268	18
Central carbohydrate metabolism
acs1	Acetyl-CoA-synthethase	39	TTGTGCTTCTCAATAATGTCCCAGTACCTTGAGAAGTTG	1263–1301	17
eno1	Enolase	25	TTACGGAAGTTGGTGCCAGCGTAGA	1277–1301	17
gpd1	Glyceraldehyde-3-P-dehydrogenase	29	ACGAAGTTGGGGTTCAGGGAGATACCAGC	895–923	17
Growth and conidiation
ccg9	Trehalose synthase	39	AAACTTTGACTTCGAACCCTTCATACGTCGACAGTTGAA	902–940	34
chs1	Chitin synthase	37	GAAAGAAGCGATAAAGTAGAGGCCGTAAATGGTAATC	2133–2169	54
con6	Conidiation related gene	31	TGCTTAGCGTTTTCCTTTGCTTCCTCCGACA	278–308	35
rpl16a	Ribosomal protein L13A, 60S subunit	27	CAACCTTCTTGCGCTCGTAGTAGGCAG	1612–1652	55
rps16b	Ribosomal protein S16A, 40S subunit	35	TGACACGGACGCGGATGTCGACGTTGGCGAACTTG	174–208	55
Proteases
aep1	Extracellular protease	25	CATGGAGGTGCCGCTAATCGTGTTT	1038–1062	56
axp1	Extracellular protease	33	AAGTTGAAGGTGGCATCCTTGATGTTTGCTTTG	933–965	56
mca1	Metacaspase, cysteine protease	25	AATACCCTGCGTGGAGTAGATGTAC	861–885	33
vpa1	Intracellular aspartic protease	27	GTGATGTCGGGGAGGGAATCACGCTTG	957–983	57
O ₂ regulated
hem6	Coproporphyrinogen III oxidase	29	ACTTCTTGAACCGAGGGTAGTACGTCTTG	798–826	39
hsp70	Heat shock protein 70	33	TTGGTGATGACAATCTTGTTGGACTTACCAGTG	1545–1577	40
Transport
ctaA	ATPase copper transport	41	ACGAGTGATTGTGCCGGTTTTGTCCAAGACGACTTTGGTAA	2309–2349	31
gap1	Amino acid transporter	33	TGATACTTCCAGGCATTGCGGAATCGGATGTGG	1335–1367	52

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ISSN: 1472-6750

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