Figure 5

Degradation of AChE proceeds at similar rates in plants expressing the optimized and non-optimized sequences. Degradation was assayed by following the loss of enzymatic activity in leaf discs in the presence of the cytosolic protein synthesis inhibitor cycloheximide (A, +CHX) or in its absence (B). Plotted are means ± SEM of individual explant samples (N = 1–8). Non-linear regression analysis was performed using the Prism software with the resultant equations. oAChES + CHX: y = 103e^-0.11X (R² = 0.78); oAChES: y = 103e^-0.03X (r² = 0.36); hAChES + CHX: y = 98e^-0.14X (r² = 0.6123); hAChES: y = 95e^-0.04X (r² = 0.4463). The differences between the regression lines obtained in the presence of CHX are not significant (unpaired t-test, P = 0.195) while all other combinations are highly significant (P < 0.0001).