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Table 3 Strains and plasmids used. KmR, CmR and ApR are kanamycin, chloramphenicol and ampicillin resistance, respectively.

From: Inhibition of hydrogen uptake in Escherichia coli by expressing the hydrogenase from the cyanobacterium Synechocystissp. PCC 6803

Strains and plasmids Genotype Source
Strains   
E. coli TG1 supE thi-1 Δ(lac-proAB) Δ(mcrB-hsdSM)5 (rK - mK -) [F' traD36 proAB lacIqZΔM15] [51]
E. coli MC4100 F- araD139 ΔlacU169 rpsL thi fla [52]
E. coli HD705 MC4100 ΔhycE; defective in large subunit of the hydrogenase 3 subunit [14]
E. coli SE1174 thi-1 leu-6 suc-10 bioA2? galT27 rpsL129 chlC3 λ- fhlA102::Tn10; defective transcription of the hyc operon and fdhF [53]
E. coli SE1497 cysC43 srl-300::Tn10 thr-1 leu-6 thi-1 proA2 galK2 ara-14 xyl-5 mtl-1 lacY1 his-4 argE3 rpsL31 tsx-33 Δ(srl-fhlA); large deletion in the 59 min region removes the hyc, hyp or fhlA genes [54]
E. coli PMD23 MC4100 ΔhypF ; defective in full maturation of hydrogenase K. T. Shanmugam
E. coli WL400 selD (CmR) [55]
E. coli BW25113 lacI q rrnB T14 ΔlacZ WJ16 hsdR514 ΔaraBAD AH33 ΔrhaBAD LD78 Yale CGSG Stock Center
E. coli BW25113 ΔhyaA Δkan E. coli JW0954 [41] Δkan; defective in small subunit of hydrogenase 1 this study
E. coli BW25113 ΔhyaB Δkan E. coli JW0955 [41]Δkan; defective in large subunit of hydrogenase 1 this study
E. coli BW25113 ΔhybB Δkan E. coli JW5494 [41] Δkan; defective in probable cytochrome Ni/Fe component of hydrogenase 2 this study
E. coli BW25113 ΔhybC Δkan E. coli JW2962 [41]Δkan; defective in probable large subunit of hydrogenase 2 this study
E. coli BW25113 ΔhycE Δkan E. coli JW2691 [41] Δkan; defective in large subunit of hydrogenase 3 this study
E. coli BW25113 ΔhycG Δkan E. coli JW2689 [41]Δkan; defective in subunit of hydrogenase 3 and formate hydrogenlyase complex this study
E. coli MW1000 BW25113 ΔhyaB ΔhybC Δkan; defective in large subunit of hydrogenase 1 and 2 this study
E. coli MW1001 BW25113 ΔhyaB ΔhybC ΔhycE Δkan; defective in large subunit of hydrogenases 1, 2, and 3 this study
E. aerogenes HU-101 Hydrogen-producing bacteria (positive control strain; wild type) NBRC
Plasmids   
pBS(Kan)Synhox pBS(Kan) plac::hoxEFUYH; KmR, expresses hydrogenase genes derived from Synechocystis sp. PCC 6803 this study
pBS(Kan)HoxU pBS(Kan) plac::hoxU; KmR, expresses HoxU derived from Synechocystis sp. PCC 6803 this study
pBS(Kan)HoxEFU pBS(Kan) plac::hoxEFU; KmR, expresses HoxEFU derived from Synechocystis sp. PCC 6803 this study
pBS(Kan)HoxFU pBS(Kan) plac::hoxFU; KmR, expresses HoxFU derived from Synechocystis sp. PCC 6803 this study
pBS(Kan)HoxFUY pBS(Kan) plac::hoxFUY; KmR, expresses HoxFUY derived from Synechocystis sp. PCC 6803 this study
pBS(Kan)HoxUYH pBS(Kan) plac::hoxUYH; KmR, expresses HoxUYH derived from Synechocystis sp. PCC 6803 this study
pCP20 ApR and CmR plasmid with temperature-sensitive replication and thermal induction of FLP synthesis [43]