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Table 2 3'/5' ratios of Housekeeping genes (internal control genes, QC2) given as average ± standard deviation.

From: Comparison of target labeling methods for use with Affymetrix GeneChips

Internal Control Genes 3'/5' Signal Ratios (Average ± SD)
Probe ID Gene Length Transcript (bases) Affy Enzo Invitro
AFFX-HUMGAPDH/M33197 GAPDH 1169 1.5 ± 0.2 1.1 ± 0.2 1.5 ± 0.3
AFFX-HSAC07/X00351 beta-Actin 1688 1.6 ± 0.2 1.5 ± 0.2 1.2 ± 0.1
AFFX-HUMRGE/M10098 18S rRNA 1795 0.9 ± 0.2 1.0 ± 0.2 0.8 ± 0.1
AFFX-M27830 28S rRNA 1816 0.03 ± 0.01* 0.02* 0*
AFFX-HUMISGF3A/M97935 ISGF3 2639 4.2 ± 1.8 3.0 ± 0.3 1.5 ± 0.1
  1. * 3' signal called absent