Table 2 3'/5' ratios of Housekeeping genes (internal control genes, QC2) given as average ± standard deviation.
From: Comparison of target labeling methods for use with Affymetrix GeneChips
Internal Control Genes | 3'/5' Signal Ratios (Average ± SD) | ||||
|---|---|---|---|---|---|
Probe ID | Gene | Length Transcript (bases) | Affy | Enzo | Invitro |
AFFX-HUMGAPDH/M33197 | GAPDH | 1169 | 1.5 ± 0.2 | 1.1 ± 0.2 | 1.5 ± 0.3 |
AFFX-HSAC07/X00351 | beta-Actin | 1688 | 1.6 ± 0.2 | 1.5 ± 0.2 | 1.2 ± 0.1 |
AFFX-HUMRGE/M10098 | 18S rRNA | 1795 | 0.9 ± 0.2 | 1.0 ± 0.2 | 0.8 ± 0.1 |
AFFX-M27830 | 28S rRNA | 1816 | 0.03 ± 0.01* | 0.02* | 0* |
AFFX-HUMISGF3A/M97935 | ISGF3 | 2639 | 4.2 ± 1.8 | 3.0 ± 0.3 | 1.5 ± 0.1 |