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Table 3 Dissociation constants of native and modified CPOs

From: Improvement of activity and stability of chloroperoxidase by chemical modification

Substrate

K d

 

50 mM citrate buffer (pH 5.0)

15% DMF

 

CPO

CA-CPO

MA-CPO

PA-CPO

CPO

CA-CPO

MA-CPO

PA-CPO

thioanisole

0.56 ± 0.04

0.05 ± 0.03

0.11 ± 0.07

0.58 ± 0.06

1.05 ± 0.07

0.07 ± 0.04

0.35 ± 0.08

0.92 ± 0.04

phenol

21.04 ± 0.82

2.78 ± 0.46

8.51 ± 1.88

23.93 ± 2.88

122.70 ± 1.26

39.78 ± 2.58

13.70 ± 1.47

83.08 ± 3.96

  1. The values represent the mean of three independent sets of experiments with SD < 5%
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BMC Biotechnology

ISSN: 1472-6750

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