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Table 3 Dissociation constants of native and modified CPOs

From: Improvement of activity and stability of chloroperoxidase by chemical modification

Substrate K d
  50 mM citrate buffer (pH 5.0) 15% DMF
  CPO CA-CPO MA-CPO PA-CPO CPO CA-CPO MA-CPO PA-CPO
thioanisole 0.56 ± 0.04 0.05 ± 0.03 0.11 ± 0.07 0.58 ± 0.06 1.05 ± 0.07 0.07 ± 0.04 0.35 ± 0.08 0.92 ± 0.04
phenol 21.04 ± 0.82 2.78 ± 0.46 8.51 ± 1.88 23.93 ± 2.88 122.70 ± 1.26 39.78 ± 2.58 13.70 ± 1.47 83.08 ± 3.96
  1. The values represent the mean of three independent sets of experiments with SD < 5%