Skip to main content

Table 2 Kinetic parameters of native and modified CPOs in 15% DMF

From: Improvement of activity and stability of chloroperoxidase by chemical modification

Enzyme Sulfoxidation Oxidation of phenol
  Km (mM) kcat (1000/min) kcat/Km (1000/mM/min) Km (mM) kcat (1000/min) kcat/Km (1000/mM/min)
CPO 6.79 ± 0.54 75.4 ± 3.0 11.1 6.69 ± 0.26 6.4 ± 0.5 1.0
CA-CPO 1.43 ± 0.07 20.4 ± 0.8 14.3 5.30 ± 0.32 6.3 ± 0.4 1.2
MA-CPO 2.50 ± 0.22 41.4 ± 1.0 16.6 5.33 ± 0.48 6.4 ± 0.5 1.2
PA-CPO 2.31 ± 0.19 26.1 ± 1.6 11.3 4.87 ± 0.29 6.8 ± 0.4 1.4
  1. The values represent the mean of three independent sets of experiments with SD < 5%