Skip to main content

Table 2 Kinetic parameters of native and modified CPOs in 15% DMF

From: Improvement of activity and stability of chloroperoxidase by chemical modification

Enzyme

Sulfoxidation

Oxidation of phenol

 

Km (mM)

kcat (1000/min)

kcat/Km (1000/mM/min)

Km (mM)

kcat (1000/min)

kcat/Km (1000/mM/min)

CPO

6.79 ± 0.54

75.4 ± 3.0

11.1

6.69 ± 0.26

6.4 ± 0.5

1.0

CA-CPO

1.43 ± 0.07

20.4 ± 0.8

14.3

5.30 ± 0.32

6.3 ± 0.4

1.2

MA-CPO

2.50 ± 0.22

41.4 ± 1.0

16.6

5.33 ± 0.48

6.4 ± 0.5

1.2

PA-CPO

2.31 ± 0.19

26.1 ± 1.6

11.3

4.87 ± 0.29

6.8 ± 0.4

1.4

  1. The values represent the mean of three independent sets of experiments with SD < 5%