Table 1 Kinetic parameters of native and modified CPOs in 50 mM citrate buffer (pH 5.0)
From: Improvement of activity and stability of chloroperoxidase by chemical modification
Enzyme | Sulfoxidation | Oxidation of phenol | ||||
|---|---|---|---|---|---|---|
| Â | Km (mM) | kcat (1000/min) | kcat/Km (1000/mM/min) | Km (mM) | kcat (1000/min) | kcat/Km (1000/mM/min) |
CPO | 1.64 ± 0.09 | 19.9 ± 0.6 | 12.1 | 0.35 ± 0.01 | 1.5 ± 0.1 | 4.3 |
CA-CPO | 1.61 ± 0.08 | 24.6 ± 2.0 | 15.3 | 0.33 ± 0.02 | 2.1 ± 0.2 | 6.4 |
MA-CPO | 1.45 ± 0.11 | 21.6 ± 0.9 | 14.9 | 0.32 ± 0.01 | 1.8 ± 0.1 | 5.6 |
PA-CPO | 1.82 ± 0.16 | 24.9 ± 1.7 | 13.7 | 0.40 ± 0.03 | 1.8 ± 0.1 | 4.5 |