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Table 1 Kinetic parameters of native and modified CPOs in 50 mM citrate buffer (pH 5.0)

From: Improvement of activity and stability of chloroperoxidase by chemical modification

Enzyme Sulfoxidation Oxidation of phenol
  Km (mM) kcat (1000/min) kcat/Km (1000/mM/min) Km (mM) kcat (1000/min) kcat/Km (1000/mM/min)
CPO 1.64 ± 0.09 19.9 ± 0.6 12.1 0.35 ± 0.01 1.5 ± 0.1 4.3
CA-CPO 1.61 ± 0.08 24.6 ± 2.0 15.3 0.33 ± 0.02 2.1 ± 0.2 6.4
MA-CPO 1.45 ± 0.11 21.6 ± 0.9 14.9 0.32 ± 0.01 1.8 ± 0.1 5.6
PA-CPO 1.82 ± 0.16 24.9 ± 1.7 13.7 0.40 ± 0.03 1.8 ± 0.1 4.5
  1. The values represent the mean of three independent sets of experiments with SD < 5%