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Figure 9 | BMC Biotechnology

Figure 9

From: Incorporation of a lambda phage recombination system and EGFP detection to simplify mutagenesis of Herpes simplex virus bacterial artificial chromosomes

Figure 9

Immunostained viral plaques of recombinant viruses derived by the BAC allele replacement technique. Virus was isolated following transfection of Vero cells with recombinant HSV2-BACs expressing A33R or B5R, titered and used to infect plates of Vero cells. Monolayers were fixed and then probed with mouse anti-HA monoclonal antibody, followed by AP-conjugated goat anti-mouse antibody. (A) B5R-expressing HSV2-BAC virus infected cells. (B) A33R-expressing HSV2-BAC virus infected cells.

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BMC Biotechnology

ISSN: 1472-6750

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