Expression of transgenes from recombinant HSV-2 BACs. Vero cells were infected with either A33R-expressing (Lanes 1–2) or B5R-expressing (Lanes 4–5) HSV2-BAC recombinant viruses. Infected cells were harvested and analyzed by Western blot using mouse anti-HA monoclonal antibody. Detection was done with an HRP-conjugated sheep anti-mouse antibody. The blot was visualized by chemiluminescence. Lane 3 – Molecular size markers (kDa).