Figure 4

Construction of an HSV-2 BAC containing att R lambda recombination sites. (A) Schematic diagram of allele replacement vector pKO5.2-UL41-attR-EGFP containing UL41 sequences flanking the attR sites on either side of the EGFP gene cassette. (B) Schematic diagram of DEST-BAC, and HSV2-BAC containing the EGFP gene flanked by lambda phage attR sites for targeting gene replacements into UL41.