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Figure 3 | BMC Biotechnology

Figure 3

From: Incorporation of a lambda phage recombination system and EGFP detection to simplify mutagenesis of Herpes simplex virus bacterial artificial chromosomes

Figure 3

Construction of HSV2-BAC ΔgD. (A) Visualization of EGFP-containing BAC colonies after transformation following the allele replacement procedure. Bacterial clones harboring mutant (ΔgD) HSV2-BAC glow green because of insertion of EGFP at the site of US6. (B) Schematic diagram of HSV2-BAC with EGFP insertion at US6(gD). pBeloBAC11 refers to the insertion of the BAC sequences into UL23 (tk) of the viral genome.

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BMC Biotechnology

ISSN: 1472-6750

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