Figure 2From: Development of a one-step embryonic stem cell-based assay for the screening of sprouting angiogenesisDouble immunostainings of embryoid bodies sprouts for CD31, von Willebrand factor (vWF) and NG2 proteoglycan. CJ7 ES cells were allowed to differentiate in the presence of angiogenic growth factors (VEGF+FGF2) added at day 0. EBs angiogenic sprouts were analyzed at day 11 of differentiation. vWF immunoreactivity (red fluorescence) located in Weibel-Palade bodies can be observed in several sprouting CD31-positive cells (green fluorescence) (upper panels). Elongated NG2 proteoglycan-positive cells (red fluorescence) can be seen close to CD31-positive cells constituting endothelial sprouts (lower panels). Scale bar = 50 μm.Back to article page