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Figure 2 | BMC Biotechnology

Figure 2

From: Development of a one-step embryonic stem cell-based assay for the screening of sprouting angiogenesis

Figure 2

Double immunostainings of embryoid bodies sprouts for CD31, von Willebrand factor (vWF) and NG2 proteoglycan. CJ7 ES cells were allowed to differentiate in the presence of angiogenic growth factors (VEGF+FGF2) added at day 0. EBs angiogenic sprouts were analyzed at day 11 of differentiation. vWF immunoreactivity (red fluorescence) located in Weibel-Palade bodies can be observed in several sprouting CD31-positive cells (green fluorescence) (upper panels). Elongated NG2 proteoglycan-positive cells (red fluorescence) can be seen close to CD31-positive cells constituting endothelial sprouts (lower panels). Scale bar = 50 μm.

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