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Figure 1 | BMC Biotechnology

Figure 1

From: Intravital imaging of fluorescent markers and FRET probes by DNA tattooing

Figure 1

Fluorescent protein expression in single cells after DNA tattooing on the skin of a living mouse. A) Using a standard tattoo machine, a pVAX vector encoding GFP (upper panel) or an empty pVAX vector (lower panel) were tattooed into the skin of mice. Confocal GFP and autofluorescence images were collected as described in the text and GFP images were corrected for autofluorescence by subtracting 0.95× the autofluorescence image. B) The in vivo emission spectra of CFP, GFP, YFP or DsRed and autofluorescence, the latter at 405 nm excitation. C) The number of GFP expressing cells counted within the same 10 mm2 skin area at different time points post tattoo, as indicated in the graph. D) Merged GFP and DsRed images of skin tattooed with a mixture of H2B-GFP- and DsRed-encoding vectors. Inset, a cell expressing both H2B-GFP and DsRed.

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