Mechanism of chimera formation during the Multiple Displacement Amplification reaction

BMC Biotechnology

Table 1 Types of chimeric DNA rearrangements

	Distance between joined segments¹	Number of chimeras
Inverted Sequences	< 10 kb	337
	> 10 kb	69
Total		406
Direct Orientation	< 10 kb	11
	> 10 kb	58
Total		69
All forms		475

¹The distance (nucleotides) in the original genomic sequence, between the 3' end of the first segment and 5' end of the second segment to be joined in the chimera.

ISSN: 1472-6750