Figure 3
Purification of HRSV/F TM -NN from allantoic fluid. (A) Allantoic fluid (10 μl) from passage 4 of defective SeV expressing HRSV/FTM- (1) compared by Western Blot with passage 4 of HRSV/FTM-NN (2). (B)Coomasie-stained SDS-PAGE of allantoic fluid from uninfected eggs (1) compared to allantoic fluid from SVec/HRSV/FTM- NN infected eggs (2), HRSV/FTM- NN after concentration and affinity chromatography (3), and after a last purification step by gel filtration (4). (C) Electron Microscopy of HRSV/FTM- NN purified from eggs or (D) from rVV infected cell supernatant. The bar denotes 50 nm.