Figure 6
From: Engineering proteinase K using machine learning and synthetic genes
Increases in proteinase K activity with and without heating. Proteinase K variants were tested from triplicate independent cultures for activity after heating at 68°C for different times: unheated (circles), 2.5 minutes (squares), 5 minutes (crosses), 7.5 minutes (triangles), 10 minutes (diamonds) and 15 minutes (open squares). A: absorbance at 405 nm of substrate incubated with wild type proteinase K, B: absorbance at 405 nm of substrate incubated with variant 3–9.