Figure 8
Antigen binding ELISA of soluble antibody fragments produced in E. coli using pOPE101 with 1 μg/well lysozyme coated per well. A 5 μM gelfiltration purified fractions of scFv, Fab and four scFab constructs were applied per well, D1.3 antibody fragments were detected using mAb anti-myc tag (1:25), the ELISA measurements were done in triplicate. B ELISA signals of serial dilutions of the different fractions of the antibody fragments.