Figure 5From: Single chain Fab (scFab) fragmentAntigen binding ELISA of soluble antibody fragments produced in E. coli using pHAL1 with 1 μg/well lysozyme coated per well. A 20 μL periplasmic fractions from production in MTPs of scFv, Fab and four scFab constructs were applied, the D1.3 antibodies were detected using mouse mAb anti-Strep-Tag (1:10000), B serial dilutions of Protein L purified equimolar amounts of scFv, Fab and scFabΔC were applied, D1.3 antibody fragments were detected using Protein L conjugated to HRP (1:10000)Back to article page