Skip to main content
Figure 4 | BMC Biotechnology

Figure 4

From: Single chain Fab (scFab) fragment

Figure 4

Antigen binding phage ELISA of phage presenting D1.3 antibody fragment variants. A 5 × 108 phage produced with M13K07, B 107 phage produced with Hyperphage. Mean values and standard deviations of three completely independent experiments are given. The absorbance of scattered light at 620 nm was subtracted from the absorbance at 450 nm. The background signal of the antigen incubated with the detection antibody mAb anti-M13 HRP was subtracted. Antigen: 100 ng/well lysozyme. The D1.3 antibody phage were detected using mAb anti-M13 conjugated with HRP.

Back to article page