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Figure 4 | BMC Biotechnology

Figure 4

From: Expression, intracellular targeting and purification of HIV Nef variants in tobacco cells

Figure 4

sp-Nef constructs are unstable in tobacco protoplasts. A: Protoplasts transfected with the indicated plasmids were pulse labelled for 2 h. Cell homogenates were subjected to immunoprecipitation with anti-FLAG antiserum, followed by SDS-PAGE and fluorography. Note the size difference between cytosolic p25 and p27 (as indicated by arrowheads) and their signal-peptide fusion counterparts. Numbers at left indicate molecular weight markers in kDa. B: Protoplasts transfected with the indicated plasmids were pulse labelled for 1 h and chased for 5 h. Cell homogenates were subjected to immunoprecipitation with anti-FLAG antiserum, followed by SDS-PAGE and fluorography. Arrowhead: aglycosylated Nef p25 (sp-p25Δg).

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