Skip to main content

Table 2 Thermal conditions in PCR analysis

From: Production of transgenic strawberries by temporary immersion bioreactor system and verification by TAIL-PCR

PCR with hptIIand virGprimers Primary TAIL PCR Secondary and tertiary TAIL PCR
95°C 3 min 95°C 3 min 95°C 3 min
25 repeats: 5 repeats: 5 repeats:
95°C 30 s 95°C 20 s 95°C 20 s
62°C 30 s 51°C 1 min 53°C 1 min
72°C 1 min 72°C 2,5 min 72°C 2,5 min
72°C 7 min 15 repeats: 15 repeats:
  95°C 20 s 95°C 20 s
  51°C 1 min 53°C 1 min
  72°C 2,5 min 72°C 2,5 min
  95°C 20 s 95°C 20 s
  51°C 1 min 53°C 1 min
  72°C 2,5 min 72°C 2,5 min
  95°C 20 s 95°C 20 s
  40°C 1 min 40°C 1 min
  72°C 2,5 min 72°C 2,5 min
  72°C 5 min 72°C 5 min