Table 2 Thermal conditions in PCR analysis
PCR with hptIIand virGprimers | Primary TAIL PCR | Secondary and tertiary TAIL PCR |
|---|---|---|
95°C 3 min | 95°C 3 min | 95°C 3 min |
25 repeats: | 5 repeats: | 5 repeats: |
95°C 30 s | 95°C 20 s | 95°C 20 s |
62°C 30 s | 51°C 1 min | 53°C 1 min |
72°C 1 min | 72°C 2,5 min | 72°C 2,5 min |
72°C 7 min | 15 repeats: | 15 repeats: |
95°C 20 s | 95°C 20 s | |
51°C 1 min | 53°C 1 min | |
72°C 2,5 min | 72°C 2,5 min | |
95°C 20 s | 95°C 20 s | |
51°C 1 min | 53°C 1 min | |
72°C 2,5 min | 72°C 2,5 min | |
95°C 20 s | 95°C 20 s | |
40°C 1 min | 40°C 1 min | |
72°C 2,5 min | 72°C 2,5 min | |
72°C 5 min | 72°C 5 min |