Production of transgenic strawberries by temporary immersion bioreactor system and verification by TAIL-PCR

BMC Biotechnology

Table 1 Primers used for PCR analysis

PCR experiment	name	sequence (5'-3')	Reference
Transgene detection	hptIIF	ACGAGCGGGTTCGGCCCATT	this work
	hptIIR	CGGCTCCGGATCGGACGATT
Agrobacterium control	virGF	GCCGACAGCACCCAGTTCAC	[51]
	virGR	CCTGCCGTAAGTTTCACCTCACC
TAIL-PCR, T-DNA specific	LB1	TTCCTAAAACCAAAATCCAG	this work
	LB2	ATTCGGCGTTAATTCAGTAC
	LB3	AAAAACGTCCGCAATGTGTT
TAIL-PCR, arbitrary^a	AD2	NGTCGASWGANAWGAA	[23]
	AD3	WGTGNAGWANCANAGA	[23]
	AD6	WGCNAGTNAGWANAAG	[52]
	AD7	AWGCANGNCWGANATA	[52]

ISSN: 1472-6750