Sequence alignments of left T-DNA junction sequences of transgenic strawberries. A: Sequence alignment of the same insertion site with three different arbitrary primers. Transgenic strawberry clone J47/2 produced specific TAIL-PCR products with three different AD primers. Sequences of these products showed one T-DNA junction site, which was primed at three different positions. Sequence alignment of the three fragments is shown together with the left T-border region of the pCAMBIA 1391Z gene transfer vector shown on the first line. Sequence homologous to pCAMBIA 1391Z is shown on bold uppercase. Duplication of 26 bp of the T-DNA border region is seen after 12 bp filler DNA fragment of unknown origin. B: Sequence alignment of the left T-DNA border DNA at different insertion sites. The three different strawberry clones showed different arrangements at the left T-DNA border regions. Two separate insertion sites from one clone (J47/1 AD2 and J47/1 AD 6) are shown. Sequence homologous to pCAMBIA 1391Z is marked bold uppercase. A homologous segment of unknown origin found in two border regions is marked yellow.