Organogenesis of transgenic strawberry in Temporary Immersion Bioreactors. A: TIB container (RITA™, Cirad, France) consisting of two compartments. The lower part holds the medium and the plant material is located on a polysulfone foam disc in the upper part of the bioreactor. B: Bioreactors in cultivation room connected to automated air pressure system. The overpressure is applied to the lower compartment through sterile filter, and the air pushes the medium to the upper part immersing the plant material. The pressure is released through another filter in the lid of the container, simultaneously renewing the atmosphere in the container. C and D: Regenerating leaf tissue from strawberry cv. Jonsok in MS medium supplemented with TDZ (2.0 mg/l) and IBA (0.5 mg/l). Regeneration occurs via short callus phase, which partly shows as red colouring of the tissue. At this stage the pieces are transferred into the selection medium. E: Strawberry plantlets developed in TIB selection with hygromycin (15 mg/l). Putative transgenic shoots remain green and the rest of the plant material is necrotic. F: Plantlets regenerating in kanamycin (50 mg/l). The plantlets are partly bleached and partly green, not giving clear indications of successful gene transfer.