Figure 3

Reactivity of immune sera towards monovalent and bivalent rEDIII proteins. (A) Antibody titers in serial dilutions of murine sera drawn 1 week after priming with rAd-Bg (blue) and 1 week following the first (red), second (green) and final (violet) boosts with pVAX-EDIII-4/2 plasmid were determined in an ELISA using the rEDIII-4/2 bivalent protein as the capture antigen. The black dashed curve indicates ELISA reactivity of control sera, drawn from rAd-C primed/pVAX1-boosted mice after the final boost. (B) Antibody titers in serial dilutions of murine sera drawn from rAd-Bg primed/pVAX-EDIII-4/2 boosted mice, one week after the final boost. ELISA was performed using rEDIII-1 (blue), rEDIII-2 (green), rEDIII-3 (red) and rEDIII-4 (violet) proteins as capture antigens. The black dashed curve indicates ELISA reactivity of control sera using a mixture of the four rEDIII proteins as capture antigen. All ELISA values in this experiment represent the average of two separate determinations.