Figure 3From: An adenovirus prime/plasmid boost strategy for induction of equipotent immune responses to two dengue virus serotypesReactivity of immune sera towards monovalent and bivalent rEDIII proteins. (A) Antibody titers in serial dilutions of murine sera drawn 1 week after priming with rAd-Bg (blue) and 1 week following the first (red), second (green) and final (violet) boosts with pVAX-EDIII-4/2 plasmid were determined in an ELISA using the rEDIII-4/2 bivalent protein as the capture antigen. The black dashed curve indicates ELISA reactivity of control sera, drawn from rAd-C primed/pVAX1-boosted mice after the final boost. (B) Antibody titers in serial dilutions of murine sera drawn from rAd-Bg primed/pVAX-EDIII-4/2 boosted mice, one week after the final boost. ELISA was performed using rEDIII-1 (blue), rEDIII-2 (green), rEDIII-3 (red) and rEDIII-4 (violet) proteins as capture antigens. The black dashed curve indicates ELISA reactivity of control sera using a mixture of the four rEDIII proteins as capture antigen. All ELISA values in this experiment represent the average of two separate determinations.Back to article page