Figure 2
From: High-throughput isolation of ultra-pure plasmid DNA by a robotic system
Plasmid yield of a robot run (A) and comparison of transfection efficiencies of plasmid DNAs isolated by the robot and a commercial kit (B). (A) pLantern plasmid DNA was extracted by the robotic system. Probes were chosen randomly from four different plates of processes A and B, 10 μl of plasmid solution per slot were applied to a 1% agarose gel and visualised by ethidium bromide staining. Marker lane: 4 μl Smart Ladder (Eurogentec). The highest band (10 kb) is 80 ng, the band at 3 kb corresponds to the lowest plasmid band and is 24 ng. (B) Transfection of column-isolated GFP plasmid DNA versus robot-isolated plasmid DNA. 293 cells were transfected with equal amounts of DNA in a 24-well format. After 30 hours phase contrast and fluorescent microphotographs were taken at a 100fold magnification with a Zeiss Axiovert S100TV equipped with a FITC-filter. Arrows denote damaged cells in the transfections.