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Figure 7 | BMC Biotechnology

Figure 7

From: A novel expression system of domain I of human beta2 glycoprotein I in Escherichia coli

Figure 7

Recombinant DI in solution is an effective inhibitor of a pathogenic monoclonal human aPL in vitro. Competitive inhibition of affinity purified IS4VH/IS4VL binding to his6tagged-DI bound to a nickel chelate plate, by recombinant DI and wild type β2GPI as inhibitors. A constant amount of antibody was mixed with varying concentrations of inhibitor and allowed to equilibrate at room temperature. For a given μmol concentration of inhibitor, DI alone is a more effective inhibitor of IS4VH/IS4VL than whole β2GPI.

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